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Here, we provide a protocol to prepare libraries for sequencing using the MiSeq instrument and basic guidelines for analysis of output data from the MiSeq sequencing run.Ĭluster generation Data analysis Illumina Next generation sequencing Sequencing-by-synthesis. Next-generation sequencing involves three basic steps: library preparation, sequencing, and data analysis. For these reasons, MiSeq has become one of the most widely used next generation sequencing platforms. It is written for students without prior knowledge in informatics or computer science. MiSeq is also a cost-effective tool for various analyses focused on targeted gene sequencing (amplicon sequencing and target enrichment), metagenomics, and gene expression studies. PlasmidID is a mapping-based, assembly-assisted plasmid identification tool that analyzes and gives graphic solution for plasmid identification. Next Generation Sequecing offers detailed explanations for NGS tools. Thus, MiSeq provides an ideal platform for rapid turnaround time. NGS Library Preparation has revolutionized genomics research and has become the standard technology for large-scale genomics and transcriptomics studies. A single run can produce output data of up to 15 Gb in as little as 4 h of runtime and can output up to 25 M single reads and 50 M paired-end reads. Combined with other technologies such as RNA extraction (RNA-Seq), enrichment for exome (Exome-seq) or other. Next-generation sequencing (NGS) is an emerging technology to determine DNA/RNA sequences for whole genome or specific regions of interest at much lower cost than traditional Sanger sequencing. It performs both single- and paired-end runs with adjustable read lengths from 1 × 36 base pairs to 2 × 300 base pairs. Next-Generation Sequencing Data Analysis. Several different platforms can be used for micro-RNA data collection and analysis ( Brown, 2013 Zhang, Chiodini, Badr, & Zhang, 2011 ). The MiSeq instrument is one of the smallest benchtop sequencers that can perform onboard cluster generation, amplification, genomic DNA sequencing, and data analysis, including base calling, alignment and variant calling, in a single run. Next-generation sequencing (massively parallel sequencing) technology for DNA and RNA analysis offers both high-speed data collection and analysis capacity previously unavailable. We can provide gene decoding and analysis for whole genome, exome. MiSeq, Illumina's integrated next generation sequencing instrument, uses reversible-terminator sequencing-by-synthesis technology to provide end-to-end sequencing solutions. Our NGS Service provides wide range of NGS applications for various research purposes.